Electrophysiological and radioisotope analysis of ion fluxes induced by salt and oxidative stress in higher plant root cells

  • Veranika V. Samokhina Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus
  • Palina V. Hryvusevich Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus
  • Anatoliy I. Sokolik Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus
  • Vadim V. Demidchik Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus
DOI: https://doi.org/10.33581/2957-5060-2022-3-14-25

Abstract

Environmental stresses induce the release of electrolytes from root cells into the extracellular space, including key inorganic and organic ions. This phenomenon is described for salinity, drought, attack of pathogens, exposure to heavy metals, oxidative stress, etc. The efflux of electrolytes is also observed during some processes of normal physiology, being part of the gravitropic response, the formation of polarity and the growth of the cell elongation. Despite the long study of this phenomenon, today its molecular mechanism remains unclear. In the present study, a combined approach based on the labeled atom method (loading isotope 86Rb+ followed by recording the parameters of its efflux from root cells), reverse genetics and the patch-clamp technique has been developed for testing the biophysical and molecular nature of the efflux of electrolytes from the root cells. It has been demonstrated that in the roots of Arabidоpsis thaliana (L.) Heynh., the K+ efflux during salinisation and oxidative stress is catalysed by K+ channels encoded by the GORK gene (guard cell outward-rectifying K+ channel), and the efflux of organic anions – by anion channels encoded by the ALMT1 gene (aluminum-activated malate transporter 1). The characteristics of the combined functioning of these systems under stress have been established.

Author Biographies

Veranika V. Samokhina, Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus

senior lecturer at the department of plant cell biology and bioengineering, faculty of biology, and researcher at the laboratory of plant physiology and biotechnology

Palina V. Hryvusevich, Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus

senior lecturer at the department of plant cell biology and bioengineering, faculty of biology, and junior researcher at the laboratory of plant physiology and biotechnology

Anatoliy I. Sokolik, Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus

PhD (biology), docent; head of the laboratory of plant physiology and biotechnology, department of plant cell biology and bioengineering, faculty of biology

Vadim V. Demidchik, Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus

сorresponding member of the National Academy of Sciences of Belarus, doctor of science (biology), docent; dean of the faculty of biology

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Published
2022-10-26
Keywords: patch-clamp, plant electrophysiology, radioisotope analysis, 86Rb, ion channel, GORK, ALMT1
Supporting Agencies This study was supported as а part of the research project «Characterisation of the passive transport of organic anions across the plasma membrane of higher plant root cells» under a grant from the Ministry of Education of the Republic of Belarus for 2022 (No. 20220676), as well as within the framework of the assignment «Regularities of the impact of cold plasma on the processes of cell signaling in higher plants» of the state program of scientific research «Convergence-2025» (No. 20211734).
How to Cite
Samokhina, V. V., Hryvusevich, P. V., Sokolik, A. I., & Demidchik, V. V. (2022). Electrophysiological and radioisotope analysis of ion fluxes induced by salt and oxidative stress in higher plant root cells. Experimental Biology and Biotechnology, 3, 14-25. https://doi.org/10.33581/2957-5060-2022-3-14-25
Issue
No 3 (2022): Experimental Biology and Biotechnology
Section
Cell Biology and Physiology

Copyright (c) 2022 Experimental Biology and Biotechnology

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