Influence of light intensity and its spectral composition on photosynthetic activity of cucumber Cucumis sativus under fusarium wilt

  • Luidmila F. Kabashnikova Institute of Biophysics and Cell Engineering, National Academy of Sciences of Belarus, 27 Akademičnaja Street, Minsk 220072, Belarus
  • Irina N. Domanskaya Institute of Biophysics and Cell Engineering, National Academy of Sciences of Belarus, 27 Akademičnaja Street, Minsk 220072, Belarus
  • Lyubov V. Pashkevich Institute of Biophysics and Cell Engineering, National Academy of Sciences of Belarus, 27 Akademičnaja Street, Minsk 220072, Belarus
  • Irina A. Dremuk Institute of Biophysics and Cell Engineering, National Academy of Sciences of Belarus, 27 Akademičnaja Street, Minsk 220072, Belarus
  • Hanna V. Martysiuk Institute of Biophysics and Cell Engineering, National Academy of Sciences of Belarus, 27 Akademičnaja Street, Minsk 220072, Belarus
  • Olga V. Molchan V. F. Kuprevich Institute of Experimental Botany, National Academy of Sciences of Belarus, 27 Akademičnaja Street, Minsk 220072, Belarus
DOI: https://doi.org/10.33581/2957-5060-2022-3-39-52

Abstract

The responses of cucumber chloroplasts of the Kustovoi variety formed under lighting of different intensity (6000 and 11 000 lx) or under LED lighting with a predominance of red light and far red light to infection with fungus Fusarium oxysporum were studied. The amount of chlorophylls and carotenoids in chloroplasts formed at low light increased in 72 h after infection, and at high light a significant increase in pigment catabolism was observed. Under fusarium wilt, the violaxanthin cycle was not involved in the conditions of the studied light range, and the photochemical activity of chloroplasts did not depend much on the level of illumination. The predominance of red light or far red light caused an increase in both chlorophylls and carotenoids content in terms of the dry mass of the leaf compared to their content in plants grown on white light. Infection on white light and red light caused an increase in the total content of chlorophylls and carotenoids, and a decrease in these parameters relative to healthy plants was noted on far red light. Fusarium wilt led to a decrease in photochemical activity and electron transport of photosystem I (by 20 %) in chloroplasts on red light and far red light. A decrease in the functional efficiency of photosystem II was also observed, most likely due to irreversible changes in pigment-protein complexes with two types of LED lighting. Various mechanisms of the response of cucumber chloroplasts to pathogen infection are discussed, depending on the light conditions of the formation of photosynthetic membranes.

Author Biographies

Luidmila F. Kabashnikova, Institute of Biophysics and Cell Engineering, National Academy of Sciences of Belarus, 27 Akademičnaja Street, Minsk 220072, Belarus

corresponding member of the National Academy of Sciences of Belarus, doctor of science (biology), docent; head of the laboratory of applied biophysics and biochemistry

Irina N. Domanskaya, Institute of Biophysics and Cell Engineering, National Academy of Sciences of Belarus, 27 Akademičnaja Street, Minsk 220072, Belarus

PhD (biology); researcher at the laboratory of applied biophysics and biochemistry

Lyubov V. Pashkevich, Institute of Biophysics and Cell Engineering, National Academy of Sciences of Belarus, 27 Akademičnaja Street, Minsk 220072, Belarus

researcher at the laboratory of applied biophysics and biochemistry

Irina A. Dremuk, Institute of Biophysics and Cell Engineering, National Academy of Sciences of Belarus, 27 Akademičnaja Street, Minsk 220072, Belarus

PhD (biology); researcher at the laboratory of plant cell biophysics and biochemistry

Hanna V. Martysiuk, Institute of Biophysics and Cell Engineering, National Academy of Sciences of Belarus, 27 Akademičnaja Street, Minsk 220072, Belarus

junior researcher at the laboratory of applied biophysics and biochemistry

Olga V. Molchan, V. F. Kuprevich Institute of Experimental Botany, National Academy of Sciences of Belarus, 27 Akademičnaja Street, Minsk 220072, Belarus

PhD (biology), docent; head of the laboratory of water exchange and photosynthesis of plants

References

  1. Arsovski AA, Galstyan A, Guseman JM, Nemhauser JL. Photomorphogenesis. The Arabidopsis Book. 2012;10:e0147. DOI: 10.1199/tab.0147.
  2. Pham Vinh Ngoc, Xu Xiaosa, Huq Enamul. Molecular bases for the constitutive photomorphogenic phenotypes in Arabidopsis. Development. 2018;145(23):dev169870. DOI: 10.1242/dev.169870.
  3. Huot B, Yao J, Montgomery BL, He SY. Growth-defense tradeoffs in plants: a balancing act to optimize fitness. Molecular Plant. 2014;7(8):1267–1287. DOI: 10.1093/mp/ssu049.
  4. Casal JJ, Balasubramanian S. Thermomorphogenesis. Annual Review of Plant Biology. 2019;70:321–346. DOI: 10.1146/annurev-arplant-050718-095919.
  5. Sowden RG, Watson SJ, Jarvis P. The role of chloroplasts in plant pathology. Essays in Biochemistry. 2018;62(1):21–39. DOI: 10.1042/EBC20170020.
  6. Genoud T, Buchala AJ, Chua N-H, Métraux J-P. Phytochrome signalling modulates the SA-perceptive pathway in Arabidopsis. The Plant Journal. 2002;31(1):87–95. DOI: 10.1046/j.1365-313x.2002.01338.x.
  7. Wang Hai, Wu Guangxia, Zhao Binbin, Wang Baobao, Lang Zhihong, Zhang Chunyi, et al. Regulatory modules controlling early shade avoidance response in maize seedlings. BMC Genomics. 2016;17:269. DOI: 10.1186/s12864-016-2593-6.
  8. Griebel T, Zeier J. Light regulation and daytime dependency of inducible plant defenses in Arabidopsis: phytochrome signaling controls systemic acquired resistance rather than local defense. Plant Physiology. 2008;147(2):790–801. DOI: 10.1104/pp.108.119503.
  9. de Wit M, Spoel SH, Sanchez-Perez GF, Gommers CMM, Pieterse CMJ, Voesenek LACJ, et al. Perception of low red : farred ratio compromises both salicylic acid- and jasmonic acid-dependent pathogen defences in Arabidopsis. The Plant Journal. 2013;75(1):90–103. DOI: 10.1111/tpj.12203.
  10. Ballaré CL. Light regulation of plant defense. Annual Review of Plant Biology. 2014;65:335–363. DOI: 10.1146/annurev-arplant-050213-040145.
  11. Cerrudo I, Caliri-Ortiz ME, Keller MM, Degano ME, Demkura PV, Ballaré CL. Exploring growth-defence trade-offs in Arabidopsis: phytochrome B inactivation requires JAZ10 to suppress plant immunity but not to trigger shade-avoidance responses. Plant, Cell & Environment. 2017;40(5):635–644. DOI: 10.1111/pce.12877.
  12. Fernández-Milmanda GL, Crocco CD, Reichelt M, Mazza CA, Kӧllner TG, Zhang T, et al. A light-dependent molecular link between competition cues and defence responses in plants. Nature Plants. 2020;6(3):223–230. DOI: 10.1038/s41477-020-0604-8.
  13. Roden LC, Ingle RA. Lights, rhythms, infection: the role of light and the circadian clock in determining the outcome of plant – pathogen interactions. The Plant Cell. 2009;21(9):2546–2552. DOI: 10.1105/tpc.109.069922.
  14. Hou Xingliang, Lee Li Yen Candy, Xia Kuaifei, Yan Yuanyuan, Yu Hao. DELLAs modulate jasmonate signaling via competitive binding to JAZs. Developmental Cell. 2010;19(6):884–894. DOI: 10.1016/j.devcel.2010.10.024.
  15. Pierik R, Ballaré CL. Control of plant growth and defense by photoreceptors: from mechanisms to opportunities in agriculture. Molecular Plant. 2021;14(1):61–76. DOI: 10.1016/j.molp.2020.11.021.
  16. Courbier S, Grevink S, Sluijs E, Bonhomme P-O, Kajala K, Van Wees SCM, et al. Far-red light promotes Botrytis cinerea disease development in tomato leaves via jasmonate-dependent modulation of soluble sugars. Plant, Cell & Environment. 2020;43(11):2769–2781. DOI: 10.1111/pce.13870.
  17. Zakurin AO, Shchennikova AV, Kamionskaya AM. [Light culture of protected soil crop production: photosynthesis, photomorphogenesis and prospects for the use of LEDs]. Fiziologiya rastenii. 2020;67(3):246–258. Russian. DOI: 10.31857/S0015330320030227.
  18. Kudelina TN, Krivobok AS, Bibikova TN, Molchan OV. Features of Arabidopsis thaliana photomorphogenesis when using LED lighting with different spectral composition. Proceedings of the National Academy of Sciences of Belarus. Biological Series. 2021;66(1):42–52. Russian. DOI: 10.29235/1029-8940-2021-66-1-42-52.
  19. Naznin MT, Lefsrud M, Gravel V, Azad MOK. Blue light added with red LEDs еnhance growth characteristics, pigments content, and antioxidant capacity in lettuce, spinach, kale, basil, and sweet pepper in a controlled environment. Plants. 2019;8(4):93. DOI: 10.3390/plants8040093.
  20. Rodriguez-Amaya DB, Kimura M. HarvestPlus handbook for carotenoid analysis. Washington: HarvestPlus; 2004. 58 p. (HarvestPlus technical monograph; 2).
  21. Shlyk AA. [Determination of chlorophyll and carotenoids in extracts of green leaves]. In: Pavlinova OA, editor. Biokhimicheskie metody v fiziologii rastenii [Biochemical methods in plant physiology]. Moscow: Nauka; 1971. p. 154–170. Russian.
  22. Krause GH, Weis E. Chlorophyll fluorescence and photosynthesis: the basics. Annual Review of Plant Physiology and Plant Molecular Biology. 1991;42:313–349. DOI: 10.1146/annurev.pp.42.060191.001525.
  23. Kramer DM, Johnson G, Kiirats O, Edwards GE. New fluorescence parameters for the determination of QA redox state and excitation energy fluxes. Photosynthesis Research. 2004;79(2):209–218. DOI: 10.1023/B:PRES.0000015391.99477.0d.
  24. Makarenko MS, Kozel NV, Usatov AV, Gorbachenko OF, Averina NG. A state of PSI and PSII photochemistry of sunflowеr yellow-green plastome mutant. Online Journal of Biological Sciences. 2016;16(4):193–198. DOI: 10.3844/ojbsci.2016.193.198.
  25. Klughammer C, Schreiber U. An improved method, using saturating light pulses, for the determination of photosystem I quantum yield via P700+-absorbance changes at 830 nm. Planta. 1994;192(2):261–268. DOI: 10.1007/BF01089043.
  26. Maslova TG, Markovskaya EF. [Modern ideas about the functioning of the violaxanthin cycle (development of D. I. Sapozhnikov’s ideas)]. Fiziologiya rastenii. 2012;59(3):472–480. Russian.
  27. Gilmore AM, Yamamoto HY. Resolution of lutein and zeaxanthin using a non-endcapped, lightly carbon-loaded C18 high-performance liquid chromatographic column. Journal of Chromatography A. 1991;543:137–145. DOI: 10.1016/S0021-9673(01)95762-0.
  28. Goltsev VN, Kalaji HM, Paunov M, Baba V, Khorachek T, Moiski Ya, et al. [The use of variable chlorophyll fluorescence to assess the physiological state of the photosynthetic apparatus of plants]. Fiziologiya rastenii. 2016;63(6):881–907. Russian. DOI: 10.7868/S0015330316050055.
  29. Petsas A, Grammatikopoulos G. Drought resistance and recovery of photosystem II activity in a Mediterranean semi-deciduous shrub at the seedling stage. Photosynthetica. 2009;47(2):284–292. DOI: 10.1007/s11099-009-0044-1.
  30. Dietzel L, Bräutigam K, Pfannschmidt T. Photosynthetic acclimation: state transitions and adjustment of photosystem stoichiometry – functional relationships between short-term and long-term light quality acclimation in plants. The FEBS Journal. 2008;275(6):1080–1088. DOI: 10.1111/j.1742-4658.2008.06264.x.
  31. Huang Wei, Zhang Shi-Bao, Cao Kun-Fang. Stimulation of cyclic electron flow during recovery after chilling-induced photoinhibition of PSII. Plant and Cell Physiology. 2010;51(11):1922–1928. DOI: 10.1093/pcp/pcq144.
  32. Wang D, Dawadi B, Qu J, Ye J. Light-engineering technology for enhancing plant disease resistance. Frontiers in Plant Science. 2022;12:805614. DOI: 10.3389/fpls.2021.805614.
Published
2022-11-15
Keywords: chlorophyll, carotenoids, photosystem I, photosystem II, light of different intensity, red light, far red light, Cucumis sativus, Fusarium oxysporum sp.
How to Cite
Kabashnikova, L. F., Domanskaya, I. N., Pashkevich, L. V., Dremuk, I. A., Martysiuk, H. V., & Molchan, O. V. (2022). Influence of light intensity and its spectral composition on photosynthetic activity of cucumber Cucumis sativus under fusarium wilt. Experimental Biology and Biotechnology, 3, 39-52. https://doi.org/10.33581/2957-5060-2022-3-39-52
Issue
No 3 (2022): Experimental Biology and Biotechnology
Section
Cell Biology and Physiology

Copyright (c) 2022 Experimental Biology and Biotechnology

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