Post-radiation changes of cytoplasmatic calcium concentration in platelets activated by ADP and thrombin

  • Olga G. Parkhimovich International Sakharov Environmental Institute, Belarusian State University, 23/1 Daŭhabrodskaja Street, Minsk 220070, Belarus
  • Olga D. Bichan Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus
  • Klavdiya Ya. Bulanova International Sakharov Environmental Institute, Belarusian State University, 23/1 Daŭhabrodskaja Street, Minsk 220070, Belarus
DOI: https://doi.org/10.33581/2957-5060-2023-1-4-13

Abstract

In the short term (3rd, 10th and 30th days) after irradiation at a dose of 1 Gy, platelets show an increased basal level of calcium ions in the cytoplasm compared to the control values, both in the presence and in the absence of calcium ions in the external environment. Under the action of ADP (20 µmol/L) and thrombin (0.02 U/mL) under similar conditions, there is an increase in the concentration of calcium ions in the cytoplasm of platelets in the post-radiation period. The most pronounced radiation-induced changes in the content of cytoplasmic calcium in resting and activated platelets are typical for the 3rd day of the post-radiation period. The data obtained indicate that the increased reactivity (resensitisation) of plasma membrane receptors in the short term after irradiation at a dose of 1 Gy creates conditions for the manifestation of hemorrhagic syndrome not only with the development of radiation sickness, but even with low-dose irradiation.

Author Biographies

Olga G. Parkhimovich, International Sakharov Environmental Institute, Belarusian State University, 23/1 Daŭhabrodskaja Street, Minsk 220070, Belarus

lecturer at the department of environmental chemistry and biochemistry, faculty of environmental medicine

Olga D. Bichan, Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus

head of the educational laboratory, faculty of physics

Klavdiya Ya. Bulanova, International Sakharov Environmental Institute, Belarusian State University, 23/1 Daŭhabrodskaja Street, Minsk 220070, Belarus

PhD (biology), docent; associate professor at the department of environmental chemistry and biochemistry, faculty of environmental medicine

References

  1. Stozharov AN, Kvitkevich LA, Solodkaya GA, Avetisov AR, Sinyakova OK, Sychik SI. Radiatsionnaya meditsina [Radiation medicine]. Stozharov AN, editor. Minsk: Minsk State Medical Institute; 2000. 154 p. Russian.
  2. Parkhimovich OG, Bichan OD, Bulanova KYa. Post-radiation changes in the content of platelets in the blood and their functional activity. Journal of the Belarusian State University. Ecology. 2022;2:59–65. Russian. DOI: 10.46646/2521-683X/2022-2-59-65.
  3. Shaturny VI, Shakhidzhanov SS, Sveshnikova AN, Panteleev MA. Activators, receptors and signal transduction pathways of blood platelets. Biomeditsinskaya khimiya. 2014;60(2):182–200. Russian.
  4. Zagidullin NSh, Valeeva KF, Gassanov N, Zagidullin ShZ. Value of endothelial dysfunction in cardiovascular diseases and methods of its correction with drugs. Kardiologiya. 2010;50(5):54–60. Russian.
  5. Clifford EE, Parker K, Humphreys BD, Kertesy SB, Dubyak GR. The P2X1 receptor, an adenosine triphosphate – gated cation channel, is expressed in human platelets but not in human blood leukocytes. Blood. 1998;91(9):3172–3181. DOI: 10.1182/blood.V91.9.3172.
  6. Offermanns S. Activation of platelet function through G protein – coupled receptors. Circulation Research. 2006;99(12):1293–1304. DOI: 10.1161/01.RES.0000251742.71301.16.
  7. Berridge MJ, Bootman MD, Roderick HL. Calcium signalling: dynamics, homeostasis and remodelling. Nature Reviews Molecular Cell Biology. 2003;4(7):517–529. DOI: 10.1038/nrm1155.
  8. Barinov EF, Sulayeva ON. The role of purine receptors of thrombocytes in regulation of hemostasis. Russian Clinical Laboratory Diagnostics. 2012;11:30–35. Russian.
  9. Kennedy C. The discovery and development of P2 receptor subtypes. Journal of the Autonomic Nervous System. 2000;81(1–3):158–163. DOI: 10.1016/s0165-1838(00)00133-8.
  10. Varga-Szabo D, Braun A, Nieswandt B. Calcium signaling in platelets. Journal of Thrombosis and Haemostasis. 2009;7(7):1057–1066. DOI: 10.1111/j.1538-7836.2009.03455.x.
  11. Léon C, Hechler B, Freund M, Eckly A, Vial C, Ohlmann P, et al. Defective platelet aggregation and increased resistance to thrombosis in purinergic P2Y1 receptor – null mice. The Journal of Clinical Investigation. 1999;104(12):1731–1737. DOI: 10.1172/JCI8399.
  12. Remijn JA, Wu Y-P, Jeninga EH, Ijsseldijk MJW, van Willigen G, de Groot PG, et al. Role of ADP receptor P2Y12 in platelet adhesion and thrombus formation in flowing blood. Arteriosclerosis, Thrombosis, and Vascular Biology. 2002;22(4):686–691. DOI: 10.1161/01.atv.0000012805.49079.23.
  13. Rozalski M, Nocun M, Watala C. Adenosine diphosphate receptors on blood platelets: potential new targets for antiplatelet therapy. Acta Biochimica Polonica. 2005;52(2):411–415. DOI: 10.18388/abp.2005_3453.
  14. Rasheed H, Tirmizi AH, Salahuddin F, Rizvi ND, Arshad M, Farooq SZ, et al. Calcium signaling in human platelet aggregation mediated by platelet activating factor and calcium ionophore. Journal of Biological Sciences. 2004;4(2):117–121. DOI: 10.3923/jbs.2004.117.121.
  15. Foster CJ, Prosser DM, Agans JM, Zhai Y, Smith MD, Lachowicz JE, et al. Molecular identification and characterization of the platelet ADP receptor targeted by thienopyridine antithrombotic drugs. The Journal of Clinical Investigation. 2001;107(12):1591–1598. DOI: 10.1172/JCI12242.
  16. Brass L, Stalker TJ. Mechanisms of platelet activation. In: Gresele P, Fuster V, López JA, Page CP, Vermylen J, editors. Platelets in hematologic and cardiovascular disorders. Cambridge: Cambridge University Press; 2008. p. 37–52.
  17. Stalker TJ, Newman DK, Ma P, Wannemacher KM, Brass LF. Platelet signaling. In: Gresele P, Born GVR, Patrono C, Page CP, editors. Antiplatelet agents. Heidelberg: Springer; 2012. p. 59–85 (Handbook of experimental pharmacology; volume 210). DOI: 10.1007/978-3-642-29423-5_3.
  18. Kahn ML, Zheng Y-W, Huang W, Bigornia V, Zeng D, Moff S, et al. A dual thrombin receptor system for platelet activation. Nature. 1998;394(6694):690–694. DOI: 10.1038/29325.
  19. De Candia E. Mechanisms of platelet activation by thrombin: a short history. Thrombosis Research. 2012;129(3):250–256. DOI: 10.1016/j.thromres.2011.11.001.
  20. Vlasenko LP, Yakutin MV. Mediating the effect of thrombin on platelets by PAR4 and PAR1 receptors. International Research Journal. 2020;8(part 2):44–47. Russian. DOI: 10.23670/IRJ.2020.98.8.040.
  21. Sambrano GR, Weiss EJ, Zheng Y-W, Huang W, Coughlin SR. Role of thrombin signalling in platelets in haemostasis and thrombosis. Nature. 2001;413(6851):74–78. DOI: 10.1038/35092573.
  22. Sage SO. The Wellcome Prize lecture. Calcium entry mechanisms in human platelets. Experimental Physiology. 1997;82(5): 807–823. DOI: 10.1113/expphysiol.1997.sp004066.
Published
2023-02-17
Keywords: platelets, irradiation, calcium metabolism, aggregation, ADP, thrombin
How to Cite
Parkhimovich, O. G., Bichan, O. D., & Bulanova, K. Y. (2023). Post-radiation changes of cytoplasmatic calcium concentration in platelets activated by ADP and thrombin. Experimental Biology and Biotechnology, 1, 4-13. https://doi.org/10.33581/2957-5060-2023-1-4-13
Issue
No 1 (2023): Experimental Biology and Biotechnology
Section
Cell Biology and Physiology

Copyright (c) 2023 Experimental Biology and Biotechnology

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