The study of activity of several antioxidant enzymes in transgenic plants Nicotiana tabacum under abiotic stress conditions

  • Kristina V. Pristupa Belarusian State University, 4 Niezaliezhnasci Avenue, Minsk 220030, Belarus https://orcid.org/0000-0003-1977-4949
  • Tatsiana A. Kukulianskaya Belarusian State University, 4 Niezaliezhnasci Avenue, Minsk 220030, Belarus
  • Elena A. Khramtsova Belarusian State University, 4 Niezaliezhnasci Avenue, Minsk 220030, Belarus

Abstract

We conducted a comparative analysis of several biochemical parameters for non-transgenic and transgenic plants Nicotiana tabacum. Plants were cultivated in heavy metal polluted soils. Transgenic plants had in their genome a bacterial acdS gene encoding the 1-aminocyclopropane-1-carboxylate deaminase (ACC deaminase). Soil treatment with salts of copper(II), chromium(VI) and lead(II) in elevated concentrations promoted induction of the acdS gene expression and an increase in ACC deaminase activity in transgenic plants. It was shown the activity of superoxide dismutase, catalase, polyphenol oxidase, ascorbate oxidase, and intensity of peroxidase oxidation processes increased in plants under abiotic stress.

Author Biographies

Kristina V. Pristupa, Belarusian State University, 4 Niezaliezhnasci Avenue, Minsk 220030, Belarus

senior lecturer at the department of biochemistry, faculty of biology

Tatsiana A. Kukulianskaya, Belarusian State University, 4 Niezaliezhnasci Avenue, Minsk 220030, Belarus

PhD (biology), docent; associate professor at the department of biochemistry, faculty of biology

Elena A. Khramtsova, Belarusian State University, 4 Niezaliezhnasci Avenue, Minsk 220030, Belarus

PhD (biology), docent; associate professor at the department of genetics, faculty of biology

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Published
2024-10-22
Keywords: antioxidant system, catalase, oxidases, enzymatic antioxidants, acdS gene, Nicotiana tabacum
How to Cite
Pristupa, K. V., Kukulianskaya, T. A., & Khramtsova, E. A. (2024). The study of activity of several antioxidant enzymes in transgenic plants Nicotiana tabacum under abiotic stress conditions. Experimental Biology and Biotechnology, 3, 22-29. Retrieved from https://journals.bsu.by/index.php/biology/article/view/6494
Issue
No 3 (2024): Experimental Biology and Biotechnology
Section
Biochemistry

Copyright (c) 2024 Experimental Biology and Biotechnology

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