Determination of NaCl- induced modifications in growth processes and induction of programmed cell death in sunflower roots

  • Viera S. Mackievic Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus https://orcid.org/0000-0002-5116-0157
  • Siarhej M. Zvanarou Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus https://orcid.org/0000-0002-5403-0235
  • Artur A. Shyker Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus https://orcid.org/0000-0002-2251-0779
  • Oksana A. Turovets Palessky Institute of Plant Growing, National Academy of Sciences of Belarus, 2 Školʼnaja Street, Kryničny 247781, Mazyr District, Homielʼ Region, Belarus https://orcid.org/0000-0001-8995-7519
  • Ihar I. Smolich Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus
  • Anatoly I. Sokolik Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus https://orcid.org/0000-0002-1513-9465
  • Vadim V. Demidchik Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus https://orcid.org/0000-0003-3765-8386

Abstract

Globally, about 30 % of all soils are damaged by salinity. Salinity leads to lower productivity of agro- and biocenosis, a decline in biodiversity and, as a result, significant economic losses. For the most important oil culture – sunflower (Helianthus annuus L.), primary reactions to salt stress are almost unexplored. This paper analyzes the impact of a wide range of NaCl concentrations on the growth of the main root of sunflower sprouts and the development of programmed cell death processes in trichoblasts. Experiments have shown that sunflower plants are relatively resistant to salinity (NaCl). They were able to grow at the level up to NaCl 250 mmol/L in the environment. Significant symptoms of programmed cell death were detected only at high concentrations of NaCl 250 –500 mmol/L. Previously undescribed in the literature, the reaction of reducing the number of symptoms of programmed cell death under150 mmol/L NaCl treatment (medium salt stress level for sunflower) was determined. An inhibitory analysis of the NaCl-induced potassium flux showed that it was only 15 % inhibited by specific potassium channel antagonists (Ba2+ ). This indicates a low proportion of potassium channels and the dominant role of non-selective cation channels in providing NaCl-induced potassium loss in Helianthus annuus L. plants.

Author Biographies

Viera S. Mackievic, Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus

postgraduate student at the department of plant cell biology and bioengineering, faculty of biology

Siarhej M. Zvanarou, Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus

postgraduate student at the department of plant cell biology and bioengineering, faculty of biology

Artur A. Shyker, Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus

student at the faculty of biology

Oksana A. Turovets, Palessky Institute of Plant Growing, National Academy of Sciences of Belarus, 2 Školʼnaja Street, Kryničny 247781, Mazyr District, Homielʼ Region, Belarus

senior researcher at the laboratory of oilseeds

Ihar I. Smolich, Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus

PhD (biology), docent; head of the department of plant cell biology and bioengineering, faculty of biology

Anatoly I. Sokolik, Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus

PhD (biology); head of the laboratory of plant physiology and biotechnology and associate professor at the department of plant cell biology and bioengineering, faculty of biology

Vadim V. Demidchik, Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus

doctor of science (biology), docent; dean of the faculty of biology

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Published
2019-10-30
Keywords: sunflower, root, growth, programmed cell death, salinity, K efflux
Supporting Agencies The work was carried out in the framework of the international project of the State Committee for Science and Technology with India «To assess the effect of abiotic stressors on the release of potassium ions from sunflower cells».
How to Cite
Mackievic, V. S., Zvanarou, S. M., Shyker, A. A., Turovets, O. A., Smolich, I. I., Sokolik, A. I., & Demidchik, V. V. (2019). Determination of NaCl- induced modifications in growth processes and induction of programmed cell death in sunflower roots. Experimental Biology and Biotechnology, 3, 13-20. https://doi.org/10.33581/2521-1722-2019-3-13-20