Quantitative determination of major nandrolone metabolite in human urine by gas chromatography/mass spectrometry

  • Pavel G. Shahoika National Anti-Doping Laboratory, 31 Liasny, Minsk region 223040, Belarus
  • Aliaksandr A. Ahabalayeu National Anti-Doping Laboratory, 31 Liasny, Minsk region 223040, Belarus
  • Olga N. Tchekhovskaya National Anti-Doping Laboratory, 31 Liasny, Minsk region 223040, Belarus
  • Yury G. Pakhadnia National Anti-Doping Laboratory, 31 Liasny, Minsk region 223040, Belarus
  • Sergey A. Beliaev National Anti-Doping Laboratory, 31 Liasny, Minsk region 223040, Belarus
  • Sergey M. Leschev Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus
DOI: https://doi.org/10.33581/2520-257X-2019-1-78-85

Abstract

Nandrolone is an anabolic androgenic steroid. The use of this substance is prohibited by World Anti-Doping Agency (WADA). In National Anti-Doping Laboratory, we have developed method for quantitative determination of major nandrolone metabolite – 19-norandrosterone in human urine by GC/MS technique (Agilent 7000). Proposed method includes sample preparation of urine samples with enzymatic hydrolysis, liquid-liquid extraction followed by derivatization step with MSTFA. Deuterated 19-norandrosterone has been used as internal standard. Total run time comprised 16 min. Lower limit of quantitation accounted for 1 ng/mL. Spiked urine samples were prepared by mixing blank urine with standard solutions of 19-norandrosterone in range 1–30 ng/mL, correlation coefficient larger than 0.99. Method was verified to
following validation parameters: selectivity, linearity, repeatability, accuracy, matrix effect, stability and robustness. Furthermore, measurement uncertainty was estimated. Thus, proposed method is able to detect threshold 19-norandrosterone in human urine and carry out its quantitation conforming WADA requirements.

Author Biographies

Pavel G. Shahoika, National Anti-Doping Laboratory, 31 Liasny, Minsk region 223040, Belarus

chemist at the anti-doping testing department

Aliaksandr A. Ahabalayeu, National Anti-Doping Laboratory, 31 Liasny, Minsk region 223040, Belarus

PhD (chemistry); leading chemist at the anti-doping testing department

Olga N. Tchekhovskaya, National Anti-Doping Laboratory, 31 Liasny, Minsk region 223040, Belarus

chemist at the anti-doping testing department

Yury G. Pakhadnia, National Anti-Doping Laboratory, 31 Liasny, Minsk region 223040, Belarus

PhD (biology), docent; head of the anti-doping testing department

Sergey A. Beliaev, National Anti-Doping Laboratory, 31 Liasny, Minsk region 223040, Belarus

director

Sergey M. Leschev, Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus

doctor of science (chemistry), full professor; professor at the department of analytical chemistry, faculty of chemistry

References

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Published
2019-02-15
Keywords: 19-norandrosterone, nandrolone, gas chromatography, mass spectrometry, doping-control, androgenic anabolic steroid, quantitative determination
How to Cite
Shahoika, P. G., Ahabalayeu, A. A., Tchekhovskaya, O. N., Pakhadnia, Y. G., Beliaev, S. A., & Leschev, S. M. (2019). Quantitative determination of major nandrolone metabolite in human urine by gas chromatography/mass spectrometry. Journal of the Belarusian State University. Chemistry, 1, 78-85. https://doi.org/10.33581/2520-257X-2019-1-78-85
Issue
No 1 (2019): Journal of the Belarusian State University. Chemistry
Section
Original Articles

Copyright (c) 2019 Journal of the Belarusian State University. Chemistry

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