Influence of 3-acetyl-5-hydroxy-2-methylindole on glioma cell proliferation and metabolism

  • Jan U. Panada Research Institute for Physical Chemical Problems, Belarusian State University, 14 Lieninhradskaja Street, Minsk 220006, Belarus https://orcid.org/0000-0002-5153-8009
  • Valeriya A. Klopava Research Institute for Physical Chemical Problems, Belarusian State University, 14 Lieninhradskaja Street, Minsk 220006, Belarus
  • Tatsiana A. Kulahava Institute for Nuclear Problems, Belarusian State University, 11 Babrujskaja Street, Minsk 220006, Belarus https://orcid.org/0000-0002-1113-7323
  • Yaroslav V. Faletrov Research Institute for Physical Chemical Problems, Belarusian State University, 14 Lieninhradskaja Street, Minsk 220006, Belarus; Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus
  • Matvey S. Horetski Research Institute for Physical Chemical Problems, Belarusian State University, 14 Lieninhradskaja Street, Minsk 220006, Belarus https://orcid.org/0000-0003-3065-6214
  • Nina S. Frolova Research Institute for Physical Chemical Problems, Belarusian State University, 14 Lieninhradskaja Street, Minsk 220006, Belarus
  • Siarhei V. Koran Republican Research and Practical Center for Epidemiology and Microbiology, 23 Filimonava Street, Minsk 220114, Belarus https://orcid.org/0000-0002-7609-7801
  • Elena G. Fomina Republican Research and Practical Center for Epidemiology and Microbiology, 23 Filimonava Street, Minsk 220114, Belarus https://orcid.org/0000-0003-3028-1176
  • Vladimir M. Shkumatov Research Institute for Physical Chemical Problems, Belarusian State University, 14 Lieninhradskaja Street, Minsk 220006, Belarus b Institute for Nuclear Problems, Belarusian State University, 11 Babrujskaja Street, Minsk 220006, Belarus; Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus
DOI: https://doi.org/10.33581/2520-257X-2022-1-43-52

Abstract

A 3-acetyl analogue of 5-hydroxyindole was synthesised and evaluated for its effects on rat C6 glioma cell functions. It was found that 3-acetyl-5-hydroxy-2-methylindole at 10 μmol/L led to a sharp reduction of mitochondrial membrane potential, induction of autophagy and decrease of proliferation of C6 glioma cells. The compound’s effect was comparable to that of rotenone, an inhibitor of cell respiration.

Author Biographies

Jan U. Panada, Research Institute for Physical Chemical Problems, Belarusian State University, 14 Lieninhradskaja Street, Minsk 220006, Belarus

junior researcher at the laboratory of biochemistry of drugs

Valeriya A. Klopava, Research Institute for Physical Chemical Problems, Belarusian State University, 14 Lieninhradskaja Street, Minsk 220006, Belarus

junior researcher trainee at the laboratory of biochemistry of drugs

Tatsiana A. Kulahava, Institute for Nuclear Problems, Belarusian State University, 11 Babrujskaja Street, Minsk 220006, Belarus

PhD (biology); leading researcher at the laboratory of nanoelectromagnetism

Yaroslav V. Faletrov, Research Institute for Physical Chemical Problems, Belarusian State University, 14 Lieninhradskaja Street, Minsk 220006, Belarus; Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus

PhD (chemistry), docent; leading researcher at the laboratory of biochemistry of drugs, Research Institute for Physical Chemical Problems, Belarusian State University, and associate professor at the department of macromolecular compounds, faculty of chemistry, Belarusian State University

Matvey S. Horetski, Research Institute for Physical Chemical Problems, Belarusian State University, 14 Lieninhradskaja Street, Minsk 220006, Belarus

junior researcher at the laboratory of biochemistry of drugs

Nina S. Frolova, Research Institute for Physical Chemical Problems, Belarusian State University, 14 Lieninhradskaja Street, Minsk 220006, Belarus

researcher at the laboratory of biochemistry of drugs

Siarhei V. Koran, Republican Research and Practical Center for Epidemiology and Microbiology, 23 Filimonava Street, Minsk 220114, Belarus

researcher at the laboratory for immunology and cell biotechnology

Elena G. Fomina, Republican Research and Practical Center for Epidemiology and Microbiology, 23 Filimonava Street, Minsk 220114, Belarus

PhD (biology); head of the laboratory for immunology and cell biotechnology

Vladimir M. Shkumatov, Research Institute for Physical Chemical Problems, Belarusian State University, 14 Lieninhradskaja Street, Minsk 220006, Belarus b Institute for Nuclear Problems, Belarusian State University, 11 Babrujskaja Street, Minsk 220006, Belarus; Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus

corresponding member of the National Academy of Sciences of Belarus, doctor of science (biology), full professor; chief researcher at the laboratory of biochemistry of drugs, Research Institute for Physical Chemical Problems, Belarusian State University, and professor at the department of macromolecular compounds, faculty of chemistry, Belarusian State University

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Published
2022-03-30
Keywords: 5-hydroxyindole, glioma, autophagy, mitochondrial transmembrane potential, rotenone
Supporting Agencies Acknowledgements. This work was supported by Belarusian Republican Foundation for Fundamental Research (grant No. X20M-113).
How to Cite
Panada, J. U., Klopava, V. A., Kulahava, T. A., Faletrov, Y. V., Horetski, M. S., Frolova, N. S., Koran, S. V., Fomina, E. G., & Shkumatov, V. M. (2022). Influence of 3-acetyl-5-hydroxy-2-methylindole on glioma cell proliferation and metabolism. Journal of the Belarusian State University. Chemistry, 1, 43-52. https://doi.org/10.33581/2520-257X-2022-1-43-52
Issue
No 1 (2022): Journal of the Belarusian State University. Chemistry
Section
Original Articles

Copyright (c) 2022 Journal of the Belarusian State University. Chemistry

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