The Study and Rehabilitation of Ecosystems

Authors

  • Irina V. Puhteeva Belarusian State University, International Sakharov Environmental Institute, Dolgobrodskaya street, 23/1, 220070, Minsk, Belarus
  • Natalya V. Gerasimovich Belarusian State University, International Sakharov Environmental Institute, Dolgobrodskaya street, 23/1, 220070, Minsk, Belarus
  • Maksim S. Matyash Belarusian State University, International Sakharov Environmental Institute, Dolgobrodskaya street, 23/1, 220070, Minsk, Belarus

Keywords:

temperature, polarity, microviscosity, annular lipids, lipid bilayer, fluorescent probe pyren, lead acetate

Abstract

The purpose of this work is to study the effect of combined action of lead acetate and low temperatures on the structural and functional characteristics of plasma membranes of thymocytes from experimental animals. The object of the study was thymocytes of experimental animals (rats). Under the influence of low temperatures on the cell, damage and changes in plasma membranes are associated with phase transitions in the lipid component. The results of the conducted studies of the combined action of lead acetate and low temperatures on the structural state of the plasma membrane of cells showed that under these conditions, the structural and functional state of the plasma membranes of rat thymus cells is modified in comparison with the separate effect of damaging factors. According to the results obtained, with the action of lead acetate on thymocytes, a reorientation of the membrane components takes place, which leads to the change in its configuration, i.e. fluidity, the processes of lipid peroxidation are also triggered, in which the content of polyunsaturated fatty acids of membrane phospholipids decreases and physicochemical properties of membranes change, which plays an important physiological role in the adaptation of various organisms to external extremes. The study of changes in the structure of thymocyte membranes at a low temperature will allow us to better understand the adaptive response and the body's response to low and ultra-low temperatures, to achieve certain successes not only in medicine but also in modern developing sciences and their directions.

Author Biographies

  • Irina V. Puhteeva, Belarusian State University, International Sakharov Environmental Institute, Dolgobrodskaya street, 23/1, 220070, Minsk, Belarus

    senior lecturer of the chair of environmental medicine and radiobiology

  • Natalya V. Gerasimovich, Belarusian State University, International Sakharov Environmental Institute, Dolgobrodskaya street, 23/1, 220070, Minsk, Belarus

    PhD (biological), associate professor; associate professor of he chair of environmental medicine and radiobiology

  • Maksim S. Matyash, Belarusian State University, International Sakharov Environmental Institute, Dolgobrodskaya street, 23/1, 220070, Minsk, Belarus

    5 years student of the faculty of environmental medicine

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Published

2018-03-18

Issue

No. 3 (2017): Journal of the Belarusian State University. Ecology

Section

The Study and Rehabilitation of Ecosystems

How to Cite

[1]
Puhteeva, I.V. et al. 2018. The Study and Rehabilitation of Ecosystems. Journal of the Belarusian State University. Ecology. 3 (Mar. 2018), 31–39.