Kotransplants based on hepatocytes and mesenchimal stem cells: characteristic, clinical application and primary assessment of the approach efficiency

Authors

  • Ala A. Symanovich Republican Sientific and Practical Center for organ and tissue transplantation, the 9th Minsk city clinical hospital, Semashko street, 8, 220116, Minsk, Belarus
  • Yauheniya A. Prymakova Republican Sientific and Practical Center for organ and tissue transplantation, the 9th Minsk city clinical hospital, Semashko street, 8, 220116, Minsk, Belarus
  • Anastasiya A. Homan Republican Sientific and Practical Center for organ and tissue transplantation, the 9th Minsk city clinical hospital, Semashko street, 8, 220116, Minsk, Belarus
  • Natallya I. Dzyadzyulya Republican Sientific and Practical Center for organ and tissue transplantation, the 9th Minsk city clinical hospital, Semashko street, 8, 220116, Minsk, Belarus
  • Katsiaryna G. Piatrouskaya Republican Sientific and Practical Center for organ and tissue transplantation, the 9th Minsk city clinical hospital, Semashko street, 8, 220116, Minsk, Belarus
  • Evgenia S. Buzuk Republican Sientific and Practical Center for organ and tissue transplantation, the 9th Minsk city clinical hospital, Semashko street, 8, 220116, Minsk, Belarus
  • Victoria V. Smol’nikova Republican Sientific and Practical Center for organ and tissue transplantation, the 9th Minsk city clinical hospital, Semashko street, 8, 220116, Minsk, Belarus
  • Aliaksei E. Shcherba Republican Sientific and Practical Center for organ and tissue transplantation, the 9th Minsk city clinical hospital, Semashko street, 8, 220116, Minsk, Belarus
  • Svetlana I. Krivenko Republican Sientific and Practical Center for organ and tissue transplantation, the 9th Minsk city clinical hospital, Semashko street, 8, 220116, Minsk, Belarus

Keywords:

hepatocytes, mesenchymal stem cells, hepatocyte-like cells, co-culture, the level of liver secretory soluble proteins, morphological characteristics, cell co-transplantation

Abstract

The results of a study on the effect of co-cultivation of mesenchymal stem cells isolated from adipose tissue and hepatogenic differentiation’ mesenchymal stem cells on the viability and functional activity of hepatocytes in a coculture are presented. It was established that a high level of secretion of liver proteins by differentiated mesenchymal stem cells and providing them with functional and metabolic properties of isolated hepatocytes was observed in the first days of cultivation (HGF (p = 0.06) and ANGPTL4 (p <0.03)). Therefore, with a longer culture, it is recommended to use undifferentiated MSCs as a scaffold. The development of liver damage is affected by variable environmental factors and lifestyle (diet, physical inertia and emotional stress). To assess the effectiveness of co-transplantation of hepatocytes and allogeneic mesenchymal stem cells of adipose tissue, a pilot clinical study was conducted in ten patients with complications of liver cirrhosis in the form of liver failure. It has been established that intraportal co-infusion of hepatocytes and allogeneic mesenchymal stem cells of adipose tissue in patients with hepatic insufficiency against cirrhosis and extensive liver resection was safe against undesirable phenomena and effective against the synthetic and metabolic function of the liver.

Author Biographies

  • Ala A. Symanovich, Republican Sientific and Practical Center for organ and tissue transplantation, the 9th Minsk city clinical hospital, Semashko street, 8, 220116, Minsk, Belarus

    doctor of laboratory diagnostics

  • Yauheniya A. Prymakova, Republican Sientific and Practical Center for organ and tissue transplantation, the 9th Minsk city clinical hospital, Semashko street, 8, 220116, Minsk, Belarus

    doctor of laboratory diagnostics

  • Anastasiya A. Homan, Republican Sientific and Practical Center for organ and tissue transplantation, the 9th Minsk city clinical hospital, Semashko street, 8, 220116, Minsk, Belarus

    junior scientific researcher

  • Natallya I. Dzyadzyulya, Republican Sientific and Practical Center for organ and tissue transplantation, the 9th Minsk city clinical hospital, Semashko street, 8, 220116, Minsk, Belarus

    chief of the laboratory

  • Katsiaryna G. Piatrouskaya, Republican Sientific and Practical Center for organ and tissue transplantation, the 9th Minsk city clinical hospital, Semashko street, 8, 220116, Minsk, Belarus

    research scientist

  • Evgenia S. Buzuk, Republican Sientific and Practical Center for organ and tissue transplantation, the 9th Minsk city clinical hospital, Semashko street, 8, 220116, Minsk, Belarus

    doctor of laboratory diagnostics

  • Victoria V. Smol’nikova, Republican Sientific and Practical Center for organ and tissue transplantation, the 9th Minsk city clinical hospital, Semashko street, 8, 220116, Minsk, Belarus

    senior researcher of the scientific departament

  • Aliaksei E. Shcherba, Republican Sientific and Practical Center for organ and tissue transplantation, the 9th Minsk city clinical hospital, Semashko street, 8, 220116, Minsk, Belarus

    PhD (medical), associate professor; departmen head

  • Svetlana I. Krivenko, Republican Sientific and Practical Center for organ and tissue transplantation, the 9th Minsk city clinical hospital, Semashko street, 8, 220116, Minsk, Belarus

    PhD (medical), associate professor; deputy chief doctor

Downloads

Published

2019-03-02

Issue

No. 2 (2018): Journal of the Belarusian State University. Ecology

Section

Medical Ecology

How to Cite

[1]
Symanovich, A.A. et al. 2019. Kotransplants based on hepatocytes and mesenchimal stem cells: characteristic, clinical application and primary assessment of the approach efficiency. Journal of the Belarusian State University. Ecology. 2 (Mar. 2019), 79–87.