Анализ физико-химических характеристик мембран клеток крови пациентов с ревматоидным артритом

Irina V. Puhteeva; Alesia V. Kaurova; Natalya V. Gerasimovich; Mark L. Levin; Ludmila A. Malkevich

Irina V. Puhteeva International Sakharov Environmental Institute, Belarusian State University
Alesia V. Kaurova International Sakharov Environmental Institute, Belarusian State University
Natalya V. Gerasimovich International Sakharov Environmental Institute, Belarusian State University https://orcid.org/0000-0002-4285-6718
Mark L. Levin International Sakharov Environmental Institute, Belarusian State University https://orcid.org/0000-0002-9775-6693
Ludmila A. Malkevich Belarusian State Medical University

Abstract

In the work reviewed and analyzed the results reflecting the physical and chemical state of the lymphocyte membranes of patients with rheumatoid arthritis. It was found that patients with rheumatoid arthritis did not show significant changes in the polarity of different areas of the membrane. At the same time discovered that annular lipid microviscosity of the plasma membrane of lymphocytes in patients with rheumatoid arthritis is reduced 2.5 times compared to control values in healthy patients, and the increased microviscosity in the general area of lipid bilayer was increased this by 25 % in relation to control values. The value of the degree of quenching of tryptophan fluorescence by pyrene in patients with rheumatoid arthritis is reduced by 35 % in relation to the control. In the membranes of red blood cells of patients with rheumatoid arthritis, there were no significant differences compared with the control values.

Author Biographies

Irina V. Puhteeva, International Sakharov Environmental Institute, Belarusian State University

senior lecturer at the department of environmental medicine and radiobiology

Alesia V. Kaurova, International Sakharov Environmental Institute, Belarusian State University

student of 4^th year at the faculty of environmental medicine

Natalya V. Gerasimovich, International Sakharov Environmental Institute, Belarusian State University

PhD (biology), docent; associate professor at the department of environmental medicine and radiobiology

Mark L. Levin, International Sakharov Environmental Institute, Belarusian State University

PhD (physics and mathematics), docent; associate professor at the department of environmental medicine and radiobiology

Ludmila A. Malkevich, Belarusian State Medical University

PhD (medicine), docent; head of the department of medical rehabilitation and physiotherapy

References

1. Алехин АИ, Денисов ЛН, Исаев ЛР и др. Аэрокриотерапия в современной медицине. Москва: [б. и.]; 2002. 287 с.
2. Насонов ЕЛ, редактор. Клинические рекомендации. Ревматологи. Москва: ГЭОТАР-Медиа; 2006. 288 с.
3. Насонов ЕЛ, Каратеев ДЕ, Чичасова НВ, Чемерис НА. Современные стандарты фармакотерапии ревматоидного артрита. Клиническая фармакология и терапия. 2005;14(1):72-75.
4. Болдырев АА, Кяйвяряйнен ЕИ, Илюха ВА. Биомембранология. Петрозаводск: Кар НЦ РАН; 2006. 226 с.
5. Клаус Дж, редактор. Лимфоциты. Методы. Москва: Мир; 1990. 400 с.
6. Заводник ИБ, Пилецкая ТП, Степуро ИИ. Осмотический и механический лизис эритроцитов человека. Биологические мембраны. 1995;12(4):400-407.
7. Добрецов ГЕ, Владимиров ЮА. Флуоресцентные зонды в исследовании биологических мембран. Москва: Наука; 1980. 320 с.
8. Гланц С. Медико-биологическая статистика. Москва: Практика; 1998. 459 с.
9. Морозова ВТ, Луговская СА, Почтарь МЕ. Эритроциты: структура, функции, клинико-диагностическое значения. Клиническая лабораторная диагностика. 2007;10:21-35.

Analysis of physicochemical characteristics of blood cell membranes of patients with rheumatoid arthritis

Abstract

Author Biographies

References