γδТ-lymphocytes in patients with IgA-nephropathy

  • Alesia V. Svirskaya International Sakharov Environmental Institute, Belarusian State University https://orcid.org/0000-0003-3742-5996
  • Darya B. Nizheharodava International Sakharov Environmental Institute, Belarusian State University
  • Kirill S. Komissarov Belarusian Medical Academy of Post-Graduate Education https://orcid.org/0000-0002-2648-0642
  • Elena I. Minchenko 1st City Clinical Hospital
  • Valery S. Pilotovich Belarusian Medical Academy of Post-Graduate Education
  • Marina M. Zafranskaya International Sakharov Environmental Institute, Belarusian State University

Abstract

Currently, IgA-nephropathy is the most common form of primary glomerulonephritis resulted in the development ofend-stage renal failure, the causes of which are not only genetic, but also environmental factors that contribute to epigeneticchanges in the both cellular and humoral immunity. The main triggers of chronic autoimmune inflammation in IgA-nephropathy are environmental factors such as industrial pollutants, infectious agents, allergens and nephrotoxic xenobioticsresulted in not only the disruption of immunoglobulin A production in mucosae but also changes in the composition ofmicrobiota and mucosal lymphoid cells, including the minor population of γδT-lymphocytes.In this study, the composition of γδT-cells subsets, their activation potential and cytotoxic activity were determined as wellas the correlation of γδT-cells immunophenotype with biochemical and histological parameters of disease progression wereestablished in patients with IgA-nephropathy. The redistribution of γδT-lymphocytes subsets in peripheral blood of patientswith IgA-nephropathy was found characterizing by a predominance of tissue-resident cells (Vδ1+ and Vδ3+T-lymphocytes) anda statistically significant decrease of Vδ2+T-cells subpopulation as compared to the control group. It was shown that γδT-lymphocytes did not demonstrate the cytotoxic activity, however, the detected increase of activated marker HLA-DR expressionon Vδ1+ and Vδ3+T-lymphocytes indicated their possible antigen-presenting role in disease pathogenesis. The obtained resultscan be used as potential biomarkers in the early diagnosis of autoimmune kidney pathology.

Author Biographies

Alesia V. Svirskaya, International Sakharov Environmental Institute, Belarusian State University

master's student at the department of immunology

Darya B. Nizheharodava, International Sakharov Environmental Institute, Belarusian State University

PhD (biology), docent; associate professor at the department of immunology; leading researcher at the department of immunology and biomedicine technology, scientific research labor

Kirill S. Komissarov, Belarusian Medical Academy of Post-Graduate Education

PhD (medicine), docent; associate professor at the department of transplantology; head of the department of nephrology, kidney replacement therapy and kidney transplantation, MSPC of surgery, transplantology and hematology

Elena I. Minchenko, 1st City Clinical Hospital

nephrologist of the highest qualification category of the nephrological

Valery S. Pilotovich, Belarusian Medical Academy of Post-Graduate Education

doctor of science (medicine), professor; professor at the department of urology and nephrology

Marina M. Zafranskaya, International Sakharov Environmental Institute, Belarusian State University

doctor of science (medicine), docent; head of the department of immunology; chief researcher at the department of immunology and biomedicine technology, scientific research laboratory

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Published
2022-01-10
Keywords: IgA-nephropathy, environmental factor, epigenetics, γδT-lymphocytes, flow cytometry, autoimmune pathology
How to Cite
Svirskaya, A. V., Nizheharodava, D. B., Komissarov, K. S., Minchenko, E. I., Pilotovich, V. S., & Zafranskaya, M. M. (2022). γδТ-lymphocytes in patients with IgA-nephropathy. Journal of the Belarusian State University. Ecology, 3, 82-89. Retrieved from https://journals.bsu.by/index.php/ecology/article/view/4493
Issue
No 3 (2021): Journal of the Belarusian State University. Ecology.
Section
Medical Ecology