Extraction of anabolic steroids with aqueous-organic mixtures from hexanic solutions

  • Sergey M. Leschev Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus
  • Yury G. Pakhadnia National Anti-Doping Laboratory, 31 Liasny, Minsk Region 223040, Belarus
  • Aliaksandr A. Ahabalayeu Center for Examinations and Tests in Health Service, 2а Tavaryski Lane, Minsk 220037, Belarus
  • Mikhail F. Zayats Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus https://orcid.org/0000-0002-8400-6359
DOI: https://doi.org/10.33581/2520-257X-2022-1-31-42

Abstract

The extraction of anabolic steroids (1,4-androstadiene-3,17-dione; 17α-methyltestosterone; 19-norethiocholanolone; 4-hydroxytestosterone; 4-androstene-3,17-dione; 5α-androstane-3β,17β-diol; dehydroepiandrosterone; calusterone; clostebol; methandienone; methyldienolone; nandrolone; testosterone; epitestosterone; tibolone; 19-norandrostenedione; 1-methylene-5α-androstan-3α-ol-17-one) by aqueous-organic mixtures from hexane solutions was studied at the temperature (20 ± 1) °C. Based on the experimental data obtained, the partition ratios of anabolic steroids were calculated, which were used to optimise the standard sample preparation procedure in the process of determining the compounds under study in biologically active dietary supplements and specialised sports nutrition. It was found that the most effective and selective extractants from hydrocarbon solutions are water-acetonitrile mixtures containing from 10 to 20 % by volume of water. An extraction technique has been developed for sample preparation of biologically active dietary supplements for subsequent gas chromatographic determination of anabolic steroids in them using a triple quadrupole mass spectrometric detector. The proposed method is characterised by a standard deviation of 10–15 % and a detection limit about 10 μg/kg of biologically active dietary supplements, which makes it possible to reliably determine impurities of prohibited anabolic steroids in them.

Author Biographies

Sergey M. Leschev, Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus

doctor of science (chemistry), full professor; professor at the department of analytical chemistry, faculty of chemistry

Yury G. Pakhadnia, National Anti-Doping Laboratory, 31 Liasny, Minsk Region 223040, Belarus

PhD (biology), docent; director

Aliaksandr A. Ahabalayeu, Center for Examinations and Tests in Health Service, 2а Tavaryski Lane, Minsk 220037, Belarus

PhD (chemistry); deputy head of the laboratory of pharmacopoeial and pharmaceutical analysis

Mikhail F. Zayats, Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus

doctor of science (chemistry), docent; head of the department of analytical chemistry, faculty of chemistry

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Published
2022-03-23
Keywords: anabolic steroids, biologically active dietary supplements, extraction, partition ratios, gas chromatographymass spectrometry
How to Cite
Leschev, S. M., Pakhadnia, Y. G., Ahabalayeu, A. A., & Zayats, M. F. (2022). Extraction of anabolic steroids with aqueous-organic mixtures from hexanic solutions. Journal of the Belarusian State University. Chemistry, 1, 31-42. https://doi.org/10.33581/2520-257X-2022-1-31-42
Issue
No 1 (2022): Journal of the Belarusian State University. Chemistry
Section
Original Articles

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