Antioxidant activity of colostrum hydrolysates and their complexes with β- and γ-cyclodextrins
Keywords:
antioxidant activity, fluorescein, colostrum hydrolysateAbstract
The antioxidant activity (AOA) of 10 samples was studied: dry colostrum, its hydrolysates with a degree of hydrolysis of 1 and 5 %, ultrafiltrates of hydrolysates obtained using filters with a separation capacity of 10 and 5 kDa, clathrates of β- and γ-cyclodextrins with peptides, as well as β- and γ-cyclodextrins. The main AOA parameters were determined from the dependences of fluorescein fluorescence intensity on the logarithm of the concentration of all samples. IC₅₀ values were in the range of 4.0–56.9 μg/ml. The samples restored fluorescein fluorescence to 74–96 % at their concentration of 0.15–0.3 mg/ml. The degree of hydrolysis and subsequent ultrafiltration, as well as complexation with cyclodextrins, influenced the increase in antioxidant activity. The maximum antioxidant activity was obtained for a sample of colostrum hydrolysate ultrafiltrate in complex with γ-cyclodextrin.
References
- Wada Y, Lönnerdal B. Bioactive peptides derived from human milk proteins – mechanisms of action. Journal of Nutritional Biochemistry. 2014;25(5): 503–514.
- Mohanty D. Antimicrobial peptides as natural bio-preservative to enhance the shelf-life of food. International Journal of Food Properties. 2016;19:837–846.
- Raikos V, Dassios T. Health-promoting properties of bioactive peptides derived from milk proteins in infant food: a review. Dairy Science and Technology. 2014;94:91–101.
- Tsabouri S., Douros K., Priftis K.N. Cow’s milk allergenicity. Endocrine, Metabolic and Immune Disorders. 2014;14(1):16–26.
- Wal J-M. Bovine milk allergenicity. Annals of Allergy, Asthma and Immunology. 2004;93(5):2–11.
- Madureira AR. Invited review: physiological properties of bioactive peptides obtained from whey proteins. Journal of Dairy Science. 2010;93(2):437–455.
- Sánchez A, Vázquez A. Bioactive peptides: a review. Journal of Food Safety. 2017;1(1):29–46.
- Cao GH, Alessio HM, Cutler RG. Oxygen-radical absorbance capacity assay for antioxidants. Free Radicals In Biology And Medicine. 1993;3(14):303–311.
- Ehlenfeldt MK., Prior RI. Oxygen radical absorbance capacity (ORAC) and phenolic and anthocyanin concentrations in fruit and leaf tissues of highbush blueberry. Journal of Agricultural and Food Chemistry. 2001;49:2222–2227.
- Wei Y. A novel H2O2-triggered anti-Fenton fluorescent pro-chelator excitable with visible light. Chemical Communications. 2009;11:1413–1415.
- Tarun EI, Dankova AV, Pyrko AN. Antioxidantnaia aktivnost hexahydroquinolonov [Antioxidant activity of hexahydroquinolones]. Journal of Belarusian State University. Ecologia. 2019;2:77–83. Russian.
