Spectrophotometrical purity and stability testing of a photosensitizer based on an indotricarbocyanine dye

  • Nikita V. Belko Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus
  • Michael P. Samtsov A. N. Sevchenko Institute of Applied Physical Problems of the Belaruain State University https://orcid.org/0000-0002-8392-7463
  • Dzmitry S. Tarasau A. N. Sevchenko Institute of Applied Physical Problems, Belarusian State University, 7 Kurčatava Street, Minsk 220045, Belarus https://orcid.org/0000-0002-5008-5976
DOI: https://doi.org/10.33581/2520-2243-2020-3-17-23

Abstract

The express method for testing purity and stability of an indotricarbocyanine dye based photosensitizer for photodynamic therapy was developed. Storage of the hydrophilic photosensitizer under unfavorable conditions resulted in contamination with a hydrophobic dye. The absorption spectrum of the hydrophobic dye was characterized by a new band at 514 nm that was absent in the absorption spectrum of the photosensitizer. There was no spectral overlap between this absorption band and the absorbance of the photosensitizer. Therefore, presence of the band at 514 nm allowed us to detect contamination of the photosensitizer substance with the hydrophobic dye. It was established that the optimal photosensitizer concentration for purity testing is ca. 0.8 mmol/L, when as little as 0.6 wt. % of the impurity can be detected. The developed method is not time consuming and requires accessible equipment only. Data obtained with this method was verified with the aid of liquid chromatography-mass spectrometry.

Author Biographies

Nikita V. Belko, Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus

postgraduate student at the department of laser physics and spectroscopy, faculty of physics.

Michael P. Samtsov, A. N. Sevchenko Institute of Applied Physical Problems of the Belaruain State University

doctor of science (physics and mathematics), docent; head of the laboratory of spectroscopy.

Dzmitry S. Tarasau, A. N. Sevchenko Institute of Applied Physical Problems, Belarusian State University, 7 Kurčatava Street, Minsk 220045, Belarus

PhD (physics and mathematics); researcher at the laboratory of spectroscopy.

References

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Published
2020-10-07
Keywords: photodynamic therapy, photosensitizers, spectrophotometry
Supporting Agencies This research has been financially supported by the state program of scientific research of the Republic of Belarus «Chemical technologies and materials» (assignment 5.23), the state program of scientific research of the Republic of Belarus «Photonics, opto- and microelectronics» (assignment 1.2.10), the Belarusian Republican Foundation for Fundamental Research (project F19M-074).
How to Cite
Belko, N. V., Samtsov, M. P., & Tarasau, D. S. (2020). Spectrophotometrical purity and stability testing of a photosensitizer based on an indotricarbocyanine dye. Journal of the Belarusian State University. Physics, 3, 17-23. https://doi.org/10.33581/2520-2243-2020-3-17-23
Issue
No 3 (2020): Journal of the Belarusian State University. Physics
Section
Optics and Spectroscopy

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