Analysis of data normalization methods for photobleaching experiments

  • Mikhail V. Antonenka Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus
  • Aliaksandr A. Halavatyi European Molecular Biology Laboratory, 1 Meierkhofshtrasse, Heidelberg 69117, Germany
  • Mikalai N. Yatskou Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus
  • Victor V. Skakun Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus

Abstract

The research of normalization methods of fluorescence recovery after photobleaching experimental data for diffusion and chemical interactions models taking into account experimental features is performed. The comparison of data normalization methods is performed, and the classification of normalization methods based on biological system’s features is first proposed. The comparison of averaging experimental data method and averaging parameter estimation method is performed. It is shown that parameter estimation values averaging rather than experimental data values averaging leds to less biased estimates of biological system’s parameters.

Author Biographies

Mikhail V. Antonenka, Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus

postgraduate student at the department of systems analysis and computer simulation, faculty of radiophysics and computer technologies

Aliaksandr A. Halavatyi, European Molecular Biology Laboratory, 1 Meierkhofshtrasse, Heidelberg 69117, Germany

PhD (physics and mathematics); researcher

Mikalai N. Yatskou, Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus

PhD (physics and mathematics); associate professor at the department of systems analysis and computer simulation, faculty of radiophysics and computer technologies

Victor V. Skakun, Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus

PhD (physics and mathematics); head of the department of systems analysis and computer simulation, faculty of radiophysics and computer technologies

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Published
2017-01-23
Keywords: photobleaching, data analysis, simulation model, fluorescence recovery curve, normalization methods, diffusion
How to Cite
Antonenka, M. V., Halavatyi, A. A., Yatskou, M. N., & Skakun, V. V. (2017). Analysis of data normalization methods for photobleaching experiments. Journal of the Belarusian State University. Physics, 1, 108-117. Retrieved from https://journals.bsu.by/index.php/physics/article/view/432
Section
Research Instruments and Methods