Electrophysical method for accelerated detection and differentiation of microorganisms based on film biostructures and planar-capacitive chip-formats

  • Aleksandr I. Drapeza Belarusian State University, Niezaliežnasci Avenue, 4, 220030, Minsk, Belarus
  • Valery A. Loban Belarusian State University, Niezaliežnasci Avenue, 4, 220030, Minsk, Belarus
  • Aleksandr I. Khmelnitsky Belarusian State University, Niezaliežnasci Avenue, 4, 220030, Minsk, Belarus
  • Gennady A. Skorokhod Belarusian State University, Niezaliežnasci Avenue, 4, 220030, Minsk, Belarus
  • Valery A. Sysov Belarusian State University, Niezaliežnasci Avenue, 4, 220030, Minsk, Belarus

Abstract

Based on the film biostructures and planar-capacitive chip-formats, an electrophysical method for the detection and differentiation of S. aureus, E. coli, P. aeruginosa and C. albicans microorganisms within 40 minutes was developed. The planar-capacitive chip-formats of the non-Faradey type sensors, with aluminum microelectrodes isolated by a dense composite film consisting of Al2O3 (200 nm) + SiO2 (100 nm), were used. The formation and measurements of the charge characteristics of film biostructures were carried out in the air at 37 °C and 5 % relative humidity. A charge of the film biostructures was determined from the area under the current-time curve after its polynomial approximation for n = 2000 and α = 99.9 %. A sample set of similar measurements was processed statistically using Excel 2007 for the number of measurements n = 3 and reliability α = 90 % on the assumption that a random variable was distributed according to the normal law. It was shown that selective populations (n = 3, α = 90 %, t = 2.4) were representative enough to detect and differentiate microorganisms, according to the informative indices of the charge of their film biostructures, with the confidence coefficient Р ≤ 0.1.

Author Biographies

Aleksandr I. Drapeza, Belarusian State University, Niezaliežnasci Avenue, 4, 220030, Minsk, Belarus

PhD (engineering), docent; head of the research laboratory of bioanalytical systems, faculty of physics

Valery A. Loban, Belarusian State University, Niezaliežnasci Avenue, 4, 220030, Minsk, Belarus

PhD (engineering); leading researcher at the research laboratory of bioanalytical systems, faculty of physics

Aleksandr I. Khmelnitsky, Belarusian State University, Niezaliežnasci Avenue, 4, 220030, Minsk, Belarus

PhD (physics and mathematics), docent; associate professor at the department of biophysics, faculty of physics

Gennady A. Skorokhod, Belarusian State University, Niezaliežnasci Avenue, 4, 220030, Minsk, Belarus

PhD (medicine), docent; head of the laboratory of intrahospital infection research

Valery A. Sysov, Belarusian State University, Niezaliežnasci Avenue, 4, 220030, Minsk, Belarus

student at the faculty of physics

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Published
2017-09-29
Keywords: electrophysical method, planar-capacitive chip-format, film biostructures, S. aureus, E. coli, P. aeruginosa, C. albicans microorganisms, detection and differentiation
How to Cite
Drapeza, A. I., Loban, V. A., Khmelnitsky, A. I., Skorokhod, G. A., & Sysov, V. A. (2017). Electrophysical method for accelerated detection and differentiation of microorganisms based on film biostructures and planar-capacitive chip-formats. Journal of the Belarusian State University. Physics, 3, 26-32. Retrieved from https://journals.bsu.by/index.php/physics/article/view/453
Issue
No 3 (2017): Journal of the Belarusian State University. Physics
Section
Physics of Biostructures

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