Comparative analysis of the dissociation rate of tetrapyrrole compounds from inclusion complexes with monomeric and polymeric cyclodextrin derivatives

  • Ivan V. Kablov Belarusian State University, 4 Niezaliezhnasci Avenue, Minsk 220030, Belarus
  • Irina E. Kravchenko Belarusian State University, 4 Niezaliezhnasci Avenue, Minsk 220030, Belarus
  • Tatiana E. Zorina Belarusian State University, 4 Niezaliezhnasci Avenue, Minsk 220030, Belarus
  • Vahab Kaskeh International Sakharov Environmental Institute, Belarusian State University, 23/1 Dawgabrodskaja Street, Minsk 220070, Belarus
  • Vladimir P. Zorin Belarusian State University, 4 Niezaliezhnasci Avenue, Minsk 220030, Belarus; International Sakharov Environmental Institute, Belarusian State University, 23/1 Dawgabrodskaja Street, Minsk 220070, Belarus

Abstract

A systematic study of the formation efficiency and dissociation rate of inclusion complexes of the tetrapyrrole photosensitiser meta-tetra(hydroxyphenyl)chlorine (mTHPC) with monomeric methyl-β-cyclodextrin (M-β-CD) and polymeric derivatives of cyclodextrin – β-cyclodextrin polymer (β-CDPS) and carboxymethyl-β-cyclodextrin polymer (CM-β-CDPS) was carried out using fluorescence methods. It is shown that a number of parameters of fluorescence excitation and emission spectra can be used to control the binding processes of photosensitiser molecules to the studied nanostructures. On the basis of the analysis of binding isotherms, the conclusion about the influence of the structure features of polymers on their interaction with mTHPC was made. The binding constants of mTHPC to β-CDPS and СM-β-CDPS were shown to be significantly higher compared the binding constant of mTHPC to M-β-CD. The study of dissociation processes of mTHPC from inclusion complexes during dilution of solutions, as well as during redistribution of photosensitiser molecules on lipid vesicles showed that the dissociation rate of mTHPC from inclusion complexes with β-CDPS and CM-β-CDPS is significantly lower than the dissociation rate of its from inclusion complexes with M-β-CD. The introduction of photosensitisers as part of inclusion complexes with polymeric cyclodextrins may provide a greater degree of control over the distribution of the drugs, as well as the ability to predict pharmacokinetic behaviour of pigment.

Author Biographies

Ivan V. Kablov, Belarusian State University, 4 Niezaliezhnasci Avenue, Minsk 220030, Belarus

postgraduate student at the department of biophysics, faculty of physics

Irina E. Kravchenko, Belarusian State University, 4 Niezaliezhnasci Avenue, Minsk 220030, Belarus

senior researcher at the laboratory of biophysics and biotechnology, department of biophysics, faculty of physics

Tatiana E. Zorina, Belarusian State University, 4 Niezaliezhnasci Avenue, Minsk 220030, Belarus

PhD (biology), docent; head of the laboratory of biophysics and biotechnology, department of biophysics, faculty of physics

Vahab Kaskeh, International Sakharov Environmental Institute, Belarusian State University, 23/1 Dawgabrodskaja Street, Minsk 220070, Belarus

postgraduate student at the department of general and medical physics, faculty of environmental monitoring

Vladimir P. Zorin, Belarusian State University, 4 Niezaliezhnasci Avenue, Minsk 220030, Belarus; International Sakharov Environmental Institute, Belarusian State University, 23/1 Dawgabrodskaja Street, Minsk 220070, Belarus

PhD (biology), docent; leading researcher at the laboratory of biophysics and biotechnology, department of biophysics, faculty of physics, Belarusian State University, and associate professor at the department of general and medical physics, faculty of environmental monitoring, International Sakharov Environmental Institute, Belarusian State University

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Published
2024-05-27
Keywords: temoporfin, photosensitiser, inclusion complexes, cyclodextrins, polymers, lipid vesicles
Supporting Agencies This work was carried out within the framework of the state programmes of scientific research «Biotechnologies-2» (assignment 1.29.2) and «Convergence-2025» (assignment 3.03.7.2).
How to Cite
Kablov, I. V., Kravchenko, I. E., Zorina, T. E., Kaskeh, V., & Zorin, V. P. (2024). Comparative analysis of the dissociation rate of tetrapyrrole compounds from inclusion complexes with monomeric and polymeric cyclodextrin derivatives. Journal of the Belarusian State University. Physics, 2, 30-37. Retrieved from https://journals.bsu.by/index.php/physics/article/view/6139
Issue
No 2 (2024): Journal of the Belarusian State University. Physics
Section
Biophysics

Copyright (c) 2024 Journal of the Belarusian State University. Physics

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