Photodynamic therapy using the photosensitizer based on tricarbocyanine dye with polyethylene glycol on a model for tumor bearing laboratory animals

  • Michael P. Samtsov aA. N. Sevchenko Institute of Applied Physical Problems, Belarusian State University, 7 Kurčatava Street, Minsk 220108, Belarus
  • Dmitriy S. Tarasov A. N. Sevchenko Institute of Applied Physical Problems, Belarusian State University, 7 Kurčatava Street, Minsk 220108, Belarus
  • Eugeni S. Voropay Belarusian State University, Niezaliežnasci Avenue, 4, 220030, Minsk, Belarus
  • Liudmila S. Lyashenko Belarusian State University, Niezaliežnasci Avenue, 4, 220030, Minsk, Belarus
  • Peter T. Petrov Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus, 5 Akademika V. F. Kupreviča Street, 2 building, Minsk 220141, Belarus
  • Vladzimir M. Nasek Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus, 5 Akademika V. F. Kupreviča Street, 2 building, Minsk 220141, Belarus
  • Aliaksandr O. Savin Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus, 5 Akademika V. F. Kupreviča Street, 2 building, Minsk 220141, Belarus
  • Raman D. Zilberman Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus, 5 Akademika V. F. Kupreviča Street, 2 building, Minsk 220141, Belarus

Abstract

Comparative studies of the effectiveness of photodynamic therapy during the experiments «in vitro» and «in vivo» have been performed when using radiation of a semiconductor laser with the wavelength 740 nm and of a light-emitting diode with the wavelength 780 nm. It has been established that the percentage of dead cancer cells is the same when the photosensitizer in HeLa cells is subjected to the photoeffect of light quanta with different energies and when absorption of the identical numbers of photons per unit time by the photosensitizer is ensured.  In the experiments with laboratory animals in vivo for a strain of M-1 sarcoma tumors it has been established that, due to an increase in the photoexposure wavelength from 740 to 780 nm and owing to the maintained the same numbers of absorbed light quanta per unit time per unit volume of the tumors, a depth of their damage increased by a factor of 1.5. The  observed changes are associated both with the differences in the transmission of tissues in vivo as the wavelength of light radiation increases and with the growing local oxygen concentration due to the photodissociation of oxyhemoglobin.

Author Biographies

Michael P. Samtsov, aA. N. Sevchenko Institute of Applied Physical Problems, Belarusian State University, 7 Kurčatava Street, Minsk 220108, Belarus

doctor of science (physics and mathematics), docent; head of the laboratory of spectroscopy

Dmitriy S. Tarasov, A. N. Sevchenko Institute of Applied Physical Problems, Belarusian State University, 7 Kurčatava Street, Minsk 220108, Belarus

researcher at the laboratory of spectroscopy

Eugeni S. Voropay, Belarusian State University, Niezaliežnasci Avenue, 4, 220030, Minsk, Belarus

doctor of science (physics and mathematics), full professor; professor at the department of laser physics and spectroscopy, faculty of physics

Liudmila S. Lyashenko, Belarusian State University, Niezaliežnasci Avenue, 4, 220030, Minsk, Belarus

PhD (physics and mathematics), docent; associate professor at the department of laser physics and spectroscopy, faculty of physics

Peter T. Petrov, Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus, 5 Akademika V. F. Kupreviča Street, 2 building, Minsk 220141, Belarus

PhD (chemistry); head of the department of pharmacology and pharmacy

Vladzimir M. Nasek, Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus, 5 Akademika V. F. Kupreviča Street, 2 building, Minsk 220141, Belarus

PhD (medicine); head of the laboratory of toxicology

Aliaksandr O. Savin, Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus, 5 Akademika V. F. Kupreviča Street, 2 building, Minsk 220141, Belarus

researcher at the laboratory of toxicology

Raman D. Zilberman, Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus, 5 Akademika V. F. Kupreviča Street, 2 building, Minsk 220141, Belarus

researcher at the laboratory of toxicology

References

  1. Ericson MB, Wennberg AM, Larkö O. Review of photodynamic therapy in actinic keratosis and basal cell carcinoma. Thera­ peutics and clinical risk management. 2008;4(1):1– 9. DOI: 10.2147/TCRM.S1769.
  2. Dougherty TJ, Gomer CJ, Henderson BW, Jori G, Kessel D, Korbelik M, et al. Photodynamic therapy. Journal of the National Cancer Institute. 1998;90(12):889 – 905. DOI: 10.1093/jnci/90.12.889.
  3. OniszczukA, Wojtunik-Kulesza KA, Oniszczuk T, Kasprzak K. The potential of photodynamic therapy (PDT) – Experimental investigations and clinical use. Biomedicine & Pharmacotherapy. 2016;83:912–929. DOI: 10.1016/j.biopha.2016.07.058.
  4. Abrahamse H, Hamblin MR. New photosensitizers for photodynamic therapy. Biochemical Journal. 2016;473(4):347–364. DOI: 10.1016/j.biopha.2016.07.058.
  5. Stranadko EPh, ArmichevAV, GeynitsAV. Light sources for photodynamic therapy. Laser medicine. 2011;15(3):63– 69. Russian.
  6. Samtsov MP (BY), LugovskiiAP (BY), Voropai ES (BY), Petrov PT (BY), LugovskiiAA(BY), Demid DI (BY), Istomin YuP (BY), authors; Belarusian State University, nauchno-issledovatel’skoe uchrezhdenie «A. N. Sevchenko Institute of Applied Physical Problems, Belarusian State University, assignee. Water-soluble indotricarbocyanine dye as a photosensitizer for photodynamic therapy of malignant tumors. Republic of Belarus 17638. 2012 July 30. Russian.
  7. LugovskiA, SamtsovM, KaplevskyK, PetrovP, VoropayE, TarasauD, etal. Novel indotricarbocyanine dyes covalently bonded to po-lyethylene glycol for theranostics. Journal of Photochemistry and PhotobiologyA.2016;316:31–36. DOI: 10.1016/j.jphotochem.2015.10.008.
  8. Samtsov MP, Rad’koAE, Kaplevskii KN, Shevchenko KA. [Laser spectrometry complex for fluorescent diagnosis of the tumor localization area]. In: Kvantovaya elektronika. Materialy V Mezhdunarodnoi nauchno­tekhnicheskoi konferentsii; 22–25 noyabrya 2004 g.; Minsk, Belarus’ [Quantum electronics. Proceedings of the V International scientific conference; 2004 November 22–25; Minsk, Belarus]. Minsk: Belarusian State University; 2004. p. 20 –24. Russian.
  9. Ermalitskii FA, Rad’koAE, Kaplevskii KN, Shevchenko KA. [Spectrometric complex for photochemotherapy with a powerful LED] [Internet]. Minsk: Academy of Public Administration under the aegis of the President of the Republic of Belarus; 2008 [cited 2017 July 21]. Available from: http://elib.bsu.by/handle/123456789/29842. Russian.
  10. Samtsov MP, Voropay ES, Kaplevsky KN, Melnikau DG, Lyashenko LS, Istomin YuP. Influence of photon energy on the ef-ficiency of photochemotherapy. Journal of Applied Spectroscopy. 2009;76(4):576–582. DOI: 10.1007/s10812-009-9223-x. Russian.
  11. Voropai ES, Samtsov MP, Kaplevskii KN, Mel’nikov DG, Lyashenko LS. Photodynamic laser therapy and diagnosis of locali-zation areas based on new types of photosensitizers. Izvestiya Rossiiskoi akademii nauk. Seriya fizicheskaya. 2007;71(1):145–149. Russian.
  12. Coutier S, Mitra S, Bezdetnaya L, Parache RM, Georgakoudi I, Foster TH, et al. Effects of Fluence Rate on Cell Survival and Photobleaching in Meta‐Tetra‐(hydroxyphenyl) chlorin – photosensitized Colo 26 Multicell Tumor Spheroids. Photochemistry and photobiology. 2001;73(3):297–303. DOI: 10.1562/0031-8655(2001)073<0297:EOFROC>2.0.CO;2.
  13. Pass HI. Photodynamic therapy in oncology: mechanisms and clinical use. JNCI: Journal of the National Cancer Institute. 1993;85(6):443– 456. DOI: 10.1093/jnci/85.6.443.
  14. Stranadko EF. Мechanism of photodynamic action. Russian Journal of Oncology. 2000;4:52–56. Russian.
  15. Hasan T, Ortel B, MoorACE, Pogue BW. Photodynamic therapy of cancer. Cancer Medicine. 1997;50:739 –751.
  16. Matcher SJ, Elwell CE, Cooper CE, Cope M, Delpy DT. Performance comparison of several published tissue near-infrared spectroscopy algorithms. Analytical biochemistry. 1995;227(1):54 – 68. DOI: 10.1006/abio.1995.1252.
  17. Taroni P, PifferiA, TorricelliA, Comellia D, Cubeddua R. In vivo absorption and scattering spectroscopy of biological tissues. Photochemical & Photobiological Sciences. 2003;2(2):124 –129. DOI: 10.1039/B209651J.
  18. LoschenovVB, KonovVI, ProkhorovAM. Photodynamic therapy and fluorescence diagnostics. Laser physics.2000;10(6):1188 –1207.
  19. Ballangrud ÅM, Barajas O, Georgousis A, Miller GG, Moore RB, McPhee MS, et al. In vivo light transmission spectra in EMT6/Ed murine tumors and Dunning R3327 rat prostate tumors during photodynamic therapy. Lasers in Surgery and Medicine. 1997;21(2):124 –133. DOI: 10.1002/(SICI)1096-9101(1997)21:2<124::AID-LSM3>3.0.CO;2-S.
  20. Zhidkova NA, Kalinina OD, Kuchin AA, Natarovskii SN, Nemkova ON, Skobeleva NB. [Application of lens raster illumina-tors reflected light microscopes]. Optiko­mekhanicheskaya promyshlennost’. 1988;8:23–24. Russian.
  21. Shreder G, Traiber Kh. Tekhnicheskaya optika [Technical optics]. Moscow: Tekhnosfera; 2006. 424 p. Russian.
  22. Asimov MM, Asimov RM, Rubinov AN. Spectrum of the effect of laser radiation on hemoglobin of the blood vessels of skin. Journal of Applied Spectroscopy. 1998;65(6):878 – 880. DOI: 10.1007/BF02675749. Russian.
Published
2019-02-10
Keywords: photosensitizer, tumor tissues, photodynamic therapy, necrosis
How to Cite
Samtsov, M. P., Tarasov, D. S., Voropay, E. S., Lyashenko, L. S., Petrov, P. T., Nasek, V. M., Savin, A. O., & Zilberman, R. D. (2019). Photodynamic therapy using the photosensitizer based on tricarbocyanine dye with polyethylene glycol on a model for tumor bearing laboratory animals. Journal of the Belarusian State University. Physics, 1, 19-26. Retrieved from https://journals.bsu.by/index.php/physics/article/view/1379
Issue
No 1 (2019): Journal of the Belarusian State University. Physics
Section
Optics and Spectroscopy

Copyright (c) 2019 Journal of the Belarusian State University. Physics

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