Recording of f luorescence from the probes based on polymethine dyes in the tissues in vivo

  • Eugene S. Voropay Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus
  • Michael P. Samtsov A. N. Sevchenko Institute of Applied Physical Problems of Belarusian State University, 7 Kurčatava Street, Minsk 220108, Belarus
  • Lyudmila S. Lyashenko Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus

Abstract

A high level of light scattering and intrinsic luminescence of biological tissues in vivo interfere with correct recording of signals from fluorescent probes in such systems. This work presents the optimized conditions for recording of polymethine dye fluorescence in the tissues in vivo. It has been found that, to lower the effect of intrinsic luminescence of biotissues when fluorescence is recorded in vivo over the spectral range 710 – 900 nm, the wavelength of laser sources used for excitation should be above 676 nm. It has been shown that the use of a СЗС-20 absorption light filter revealing no luminescence over the spectral range under study is optimal to lower in a spectrometer a level of scattered light from the excitation laser (l = 682 nm) by three orders. By scanning an optical fiber over the surface of the tumor node, it has been demonstrated that in the case of necrotic tissues the fluorescence signal of polymethine dye in this region is lower by a factor of 3–5 as compared to other parts of the tumor.

Author Biographies

Eugene S. Voropay, Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus

doctor of science (physics and mathematics), full professor; head of the department of laser physics and spectroscopy, faculty of physics

Michael P. Samtsov, A. N. Sevchenko Institute of Applied Physical Problems of Belarusian State University, 7 Kurčatava Street, Minsk 220108, Belarus

doctor of science (physics and mathematics); head of the laboratory of spectroscopy

Lyudmila S. Lyashenko, Belarusian State University, 4 Niezaliežnasci Avenue, Minsk 220030, Belarus

PhD (physics and mathematics); associate professor at the department of laser physics and spectroscopy, faculty of physics.

References

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Published
2017-01-23
Keywords: polymethine dyes, fluorescence recording, in vivo, photoactivity
How to Cite
Voropay, E. S., Samtsov, M. P., & Lyashenko, L. S. (2017). Recording of f luorescence from the probes based on polymethine dyes in the tissues in vivo. Journal of the Belarusian State University. Physics, 1, 28-33. Retrieved from https://journals.bsu.by/index.php/physics/article/view/421